Part:BBa_K814011
scytonemin biosynthetic pathway, scyBAC
Our research has focused on novel biosynthetic pathways for UV-protectant compounds found in two distinct algal species. One leading to the production of the unrelated UV-screening compound scytonemin was cloned from Nostoc punctiforme. ScyB first converts tryptophan into 3-indole pyruvic acid, which is converted into a ketoacid intermediate by ScyA. ScyC then converts the ketoacid into an indole compound which spontaneously forms scytonemin.
This part is made of three basic parts and contains the scyB, scyA and scyC generators. This part could be used for scytonemin biosynthesis in E. coli
Figure 1. Cell pellets of control and ScyBAC expressing E. coli. Note the brown color indicative of scytonemin production as previously reported by Balskus et al., 2011.
Balskus and Walsh, 2009. [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2766776/] Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 849
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Illegal AgeI site found at 3265 - 1000COMPATIBLE WITH RFC[1000]
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